Extracellular Flux Analyzers are available for respirometry assays in intact cells and spheroids, permeabilized cells, and isolated mitochondria. These assays can be used to quantify a wide range of mitochondrial bioenergetics parameters and are used to decipher the mechanism of mitochondrial bioenergitic function in a 96 well format in real time.
High content imaging is available for the measurement of mitochondrial membrane potential, mitochondrial mass, and reactive oxygen species. Additionally, the Operetta imaging system can be scheduled and used for other customizable imaging applications outside of assessing mitochondrial function.
Experts in a vast range of topics related to mitochondrial function are available to provide consulting related to study design, troubleshooting technical problems, method development, and data interpretation.
Training is routinely provided for use of both the Extracellular Flux Analyzers and the High Content Imaging System. Additionally, guidance on assay design, data analysis and interpretation is provided for users.
The Core can undertake every aspect of the performing respirometry and imaging assays. Core staff will culture cells or isolate mitochondria, prepare and perform compound treatments, run assays, analyze data and provide results in a comprehensive report.
The Mitochondria and Metabolism Core can assist in proper study-design and data interpretation as well as ensuring that studies are properly designed and executed in a timely manner.
Contact for more information:
University of California, Los Angeles
Center for Health Sciences (CHS) 33-131
650 Charles E. Young Drive South
Los Angeles, CA 90095
lstiles@mednet.ucla.edu
(310) 825-8630
