Full Services

Metabolism Core: Full Services

Full Services Available

Respirometry

Extracellular Flux Analyzers are available for respirometry assays in intact cells and spheroids, permeabilized cells, and isolated mitochondria. Additionally, a frozen tissue respirometry assay is now available to measure maximal respiratory capacity in frozen samples.  These assays can be used to quantify a wide range of mitochondrial bioenergetics parameters in real time in a microplate. The Mitochondria and Metabolism Core provides in-depth training and scheduled use of instruments as well as full-service options for assay design, performance, and data analysis.

Imaging

High content imaging as well as high-resolution imaging are available for measurements of mitochondrial morphology and membrane potential, reactive oxygen species, mitophagy, NAD/NADH, cristae imaging, pH measurements, cell-monolayer electron microscopy, cell growth and differentiation, and more. Additionally, use of equipment can be scheduled, and training is provided.

Consultation

Experts in a vast range of topics related to mitochondrial function are available to provide consulting related to study design, troubleshooting technical problems, method development, and data interpretation.

Training

Training is routinely provided for use of both the Extracellular Flux Analyzers and the High Content Imaging System. Additionally, guidance on assay design, data analysis and interpretation is provided for users.