The Mitochondrial and Metabolism Core.

Facilitating mitochondrial bioenergetics research

The Mitochondrial and Metabolism Core supports mitochondrial research for both academic and industry users. The Core specializes in mitochondrial bioenergetics, providing guidance and services focused on mitochondrial bioenergetic functions, including respirometry and ATP production, as well as imaging of mitochondrial mass, membrane potential and reactive oxygen species. In addition to full service offerings and technical training, the Mitochondria and Metabolism Core is committed to providing technological resources as well as educational opportunities to further mitochondrial awareness and knowledge to the scientific community.

Benefits of using the Mitochondra and Metabolism Core

Measurement of mitochondrial function is widely used to explore the mechanistic basis of disease, compound treatment, or genetic alterations. Assessment of mitochondrial function is also a key component in identifying toxicity in response to treatment. While mitochondrial function is critical in understanding cellular function, model system phenotypes, and compound toxicities/mechanisms of action, it is also a complex area of study that has only become conventional in the past decade or so, which means demand for mitochondrial research has increased but expertise in the area is still limited.

Core Proclamation Statement (PDF)

How we can help:

  • Evaluation
  • Study plan design
  • Running assays
  • Interpretation of data
  • Comprehensive report
  • Future direction/guidance

The Core Assays

Mitochondrial research is complicated and can often results in poor study design and misinterpretation of data without previous experience. The Mitochondria and Metabolism Core can assist in proper study-design and data interpretation as well as ensuring that studies are properly designed and executed in a timely manner. Additionally, the Core provides access to and training on instruments that are critical to assess mitochondrial function, but that are too costly for every lab to own or are better suited as a shared resource. Our mission is that the Mitochondria and Metabolism Core will allow for mitochondrial research to completed and published appropriately, faster, and in a more cost-effective manner.
Visit the UCLA Metabolomics

UCLA Metabolomics Center shared resources

Instrumentation and Core Resources

Operetta-Imaging

PerkinElmer Operetta High Content Imaging System
Quantity: 1

Seahorse

XF96 Seahorse Extracellular Flux Analyzer
Quantity: 3

Operetta High Content Imager (PerkinElmer)
Allows investigators to conduct larger, more complex imaging experiments. Whether you are performing assay development, genome-wide siRNA, compound screens or looking at a few samples in great detail, and whether you are using 3D disease models, primary cells, stem cells, live or fixed cells, the Operetta High Content Imaging System delivers all the features you need to generate unbiased, statistically significant data to move your research forward.

XF96 Extracellular Flux Analyzer (Seahorse/Agilent)
The Seahorse XFe96 Analyzer provides the highest capacity for XF assays at the lowest per-sample cost. The Seahorse XFe96 enables a large number of experimental groups per assay, making it ideal for labs seeking to maximize design flexibility and sample throughput. The Seahorse XFe96 Analyzer can analyze individual spheroids with the Seahorse XFe96 Spheroid Microplate.

Contact Information

Lindsey Stiles

Linsey Stiles, PhD
Scientific Director
lstiles@mednet.ucla.edu
(310) 825-8630

Orian Shirihai, MD, PhD

Orian Shirihai, MD, PhD
Director
oshirihai@mednet.ucla.edu